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Abstract
An amino substituted silica gel column was used for the normal phase HPLC separation of nucleic acid components, including free bases and the corresponding 2′-deoxyribo- and ribonucleosides. The chromatographic system was also found to be particularly convenient for the separation of various modified nucleosides, including oxidation products of 2′-deoxyguanosine and pyrimidine components, thymidine cyclobutane dimers, dinucleoside monophosphate photoproducts and pyrimidine (6-4) pyrimidone adducts.