Separation of the Diastereomers of Baclofen by High Performance Liquid Chromatography Using Cyclodextrin as A Mobile Phase Additive

Abstract

Baclofen (4-amino-3-p-chlorophenylbutyric acid), a skeletal muscle relaxant used in the treatment of spastic disorders, is administered clinically as a racemic mixture. This paper describes the separation of the diastereomers of baclofen, which was derivatized with (+)-l-(9-fluorenyl)ethyl chloroformate, (+)-l-(l-naphthyl)ethyl isocyanate, 1-fluoro-2, 4-dinitrophenyl-5-L-alanine amide or 2, 3, 4, 6-tetra-β-acetyl-β-D-glucopyranosyl isothiocyanate, by reversed-phase high-performance liquid chromatography using cyclodextrin as a mobile phase additive.