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A procedure for the separation of positionally isomeric triacylglycerols by silver ion thin-layer chromatography at ambient temperature is described. The low silver ion content in the silica gel layer, the mobile phase of chloroform-methanol and the development in open cylindrical tanks are found essential for the improved resolution of the SMS-SSM, SMM-MSM, SDS-SSD species. Resolution of the DSD-DDS and DMD-DDM species is achieved also.
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