HPLC Determination of Triacylglycerols in the Digestive Gland-Gonad Complex of Biomphalaria Glabrata Snails Fed Hen's Egg Yolk Versus Leaf Lettuce

Abstract

HPLC was used to analyze triacylglycerols in the digestive gland-gonad (DGG) complex of the planorbid snail Biomphalaria glabrata fed either hen's egg yolk or leaf lettuce. Both lettuce and yolk samples were also analyzed for their triacylglycerol composition. Identification of specific triacylglycerols was made according to retention times that corresponded to those of triacylglycerol standards. Triacylglycerols identified in the hen's egg yolk were rac-glyceryl-1,2-oleate-3-stearate (OOS), trilinolein, and rac-glyceryl-1,3-palmitate-2-oleate (POP). Triacylglycerols from snails fed leaf lettuce were identified as rac-glyceryl-l-oleate-2,3-stearate (OSS), OOS, trilinolein, and rac-glyceryl-l-oleate-2,3-palmitate (OPP), whereas those from snails fed hen's egg yolk were identified as OSS, trilinolein, PSO, and POP.