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Original Articles

Pulsed UV Laser-Induced Fluorescence Detection of Native Peptides and Proteins in Capillary Electrophoresis

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Pages 1877-1890 | Published online: 23 Sep 2006
 

Abstract

A pulsed UV laser operating at 248 nm was used for the laser-induced fluorescence (LIF) detection of native tryptophan-containing compounds in capillary electrophoresis. The limit of detection (LOD) of tryptophan was found to be 3.3×10−9 M (S/N=2). The LODs of the model proteins conalbumin and bovine serum albumin were found to be 1.3×10−9 and 4×10−9 M (S/N=2), respectively. These results were at least two orders of magnitude more sensitive when compared to UV absorption at 214 nm.

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