Capillary Electrophoresis of Multiple Antigenic-Peptide (MAPS) of the Malaria Circumsporozoite Rotein Epitopes

Abstract

Capillary electrophoresis (CE) was evaluated as a means of analyzing the homogeneity of synthetic malaria vaccines consisting of multiple-antigenic-peptides (MAPs). The MAPs consist of a branching oligomeric lysine core (Lys_n, n = 3, 7, 15) with antigenic peptides coupled to each of the α-amino and ∊-amino arms (n+1 peptides total). In this report we compare CE with reversed-phase and size-exclusion liquid chromatography (RP-HPLC and SE-HPLC) for the analysis of Lys, MAPs corresponding to B-cell and T-cell epitopes of the circumsporozoite protein of the Plasmodium falciparum malaria parasite.