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Abstract
Microbore columns (1 and 2. 1 mm I. D.) were investigated for their utility in fast liquid chromatography of proteins and peptides. Using a series of SynChropak large pore reversed phase columns, the effects of flowrate and gradient on resolution of standard protein and peptide mixtures were determined. Excellent resolution could be achieved on 2.1 mm I. D. columns with analysis times under five minutes.