Resolution of the Non-Specific Spectra of Barbiturates by UV-Photodiode Array Detection. II. Effects of Sample Concentration on Spectral Matching Accuracy

Abstract

The procedure described uses a photodiode array detector for the qualitative determination of peak identity. The object was to determine the capability of a photodiode array detector system to accurately discriminate among the non-specific spectra of barbiturates at concentrations down to 1 μg/ml. Five barbiturates, all of which possessed highly similar non-specific spectra were analyzed and spectrally compared. Standard spectra for each barbiturate were created using a concentration of approximately 0.5 mg/ml. Samples of each barbiturate were prepared at concentrations down to 1 μg/ml. Spectra from these samples are compared to the standard spectra library. The photodiode array detector system accurately identified all of the barbiturate samples at all concentrations tested.