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Original Articles

Simultaneous Isocratic HPLC Separation of the Diastereoisomers of Caffeic, Ferulic, and Isoferulic Acids and Related Coumarins

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Pages 149-160 | Received 01 Apr 1992, Accepted 20 Apr 1992, Published online: 23 Sep 2006
 

Abstract

A general procedure for the simultaneous analysis of phenolic compounds by a reversed-phase high performance liquid chromatography (RP-HPLC) is described. The series of compounds embraces the cis/trans isomers of caffeic, ferulic and isoferulic acids and the coumarins - esculetin, scopoletin and isoscopoletin. In order to establish the experimental conditions, leading to an optimal resolution, several test mixtures were analysed. HPLC separation was carried out on a reversed-phase column (Lichrosorb RP-8, 5 μg), using aqueous tetrahydrofuran as a mobile phase.

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