11
Views
7
CrossRef citations to date
0
Altmetric
Original Articles

A Multi-Dimensional Liquid Chromatography Method for Determination of Androgen Hormone Residues in Cattle Liver

&
Pages 241-255 | Received 20 May 1992, Accepted 03 Jun 1992, Published online: 23 Sep 2006
 

Abstract

A multidimensional HPLC method is described for the determination of trenbolone and 19-nortestosterone steroid hormone residues in cattle liver. Sample extracts are defatted by size exclusion chromatography. The evaporated residue is injected onto a high performance gel permeation column and the two fractions containing the steroids are diverted onto individual silica concentration columns, where the hormone residues are retained. The trapped hormones are then determined separately. Trenbolone held on one concentration column is eluted onto a cyano HPLC column and the steroid peak from this is transferred onto a final analytical silica HPLC column. After re-equilibration of the system the second concentration column is treated similarly for determination of 19-nortestosterone. Detection is by UV at 340 nm for trenbolone and 247 nm for 19-nortestosterone. Detection limits in cattle liver are 0.1 μg/kg for trenbolone and 0.3 μg/kg for 19-nortestosterone. For liver spiked at 2 μg/kg. recoveries of 59% and 39%, with CVs of 7% and 10% were obtained for trenbolone and 19-nortestosterone respectively. The low overall recovery for 19-nortestosterone was primarily attributable to losses during extraction.

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.