Retention Studies of Alkyl- and Halogen-Substituted Aromatics on Normal-Phase Silica and Alumina Columns. IV. Effect of the Mobile Phase Structure on Retention Data

Abstract

Normal-phase liquid chromatography has been applied to five series of compounds: chlorobenzenes, chloronaphthalenes, chlorobiphenyls, methylbenzenes and methylbiphenyls. Experiments were performed on silica with n-octane, n-decane, iso-octane and cyclo-hexane as the mobile phases.

The data obtained are compared with previously published n-hexane retention data. Mutual differences are significant and they are apparently associated with differences in bulk and mobility of the molecules cf tha eluent. more over, the substitution pattern in the investigated series seems to be of due importance.