High-Performance Liquid Chromatographic Determination of Zinc Pyrithione in Antidandruff Shampoos Using On-Line Copper Chelate Formation

K. Nakajima Public Health Laboratory of Chiba Prefecture, 666-2, Nitona-cho, Chiba, 280, Japan; Klsarazu Public Health Center of Chiba Prefecture, 3-4-34, Shinden, Kisarazu, 292, Japan

M. Ohta Public Health Laboratory of Chiba Prefecture, 666-2, Nitona-cho, Chiba, 280, Japan; Klsarazu Public Health Center of Chiba Prefecture, 3-4-34, Shinden, Kisarazu, 292, Japan

H. Yazaki Public Health Laboratory of Chiba Prefecture, 666-2, Nitona-cho, Chiba, 280, Japan; Klsarazu Public Health Center of Chiba Prefecture, 3-4-34, Shinden, Kisarazu, 292, Japan

H. Nakazawa Department of Pharmaceutical Sciences, National Institute of Public Health, 4-6-1, Shirokane-dai, Minato-ku, Tokyo, 108, Japan; Klsarazu Public Health Center of Chiba Prefecture, 3-4-34, Shinden, Kisarazu, 292, Japan

Abstract

A rapid and simple high-performance liquid chromatographic (HPLC) method using on-line copper chelate formation was developed for the determination of zinc pyrithione (ZPT) in antidandruff shampoos. ZPT dispersed In samples was dissolved in 1 M hydrochloric acid followed by purification with polyamide microcolumn. HPLC was carried out on a Chromatorex ODS column (25 cm × 4.6 mm I. D.) using 15 % methanol containing 1 % of copper (II) nitrate trihydrate and 1% of citric acid monohydrate as the mobile phase with UV detection at 320 nm. The calibration graph was linear from 20-50 ng for ZPT. The method gave high reproducibility with coefficients of variation less than 1 %.

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High-Performance Liquid Chromatographic Determination of Zinc Pyrithione in Antidandruff Shampoos Using On-Line Copper Chelate Formation

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High-Performance Liquid Chromatographic Determination of Zinc Pyrithione in Antidandruff Shampoos Using On-Line Copper Chelate Formation

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