Cellulosic Chiral Stationary Phase Under Reversed-Phase Condition

Abstract

Chiral stationary phases on polysaccharide esters have been mainly applied under a normal phase condition. However, there are some samples that can not be analyzed under normal phase conditions because of the solubility and the procedures by which they are prepared. We have established reversed-phase conditions of mobile phases to attain good chiral separations on cellulose-based-columns. A simple mixture of water and an organic solvent as the mobile phase gave sufficient separation of an electrically neutral racemate. On the other hand, it was necessary to add an anionic chaotrope for the separation of a basic racemate and a small amount of a strong acid for an acidic one.