Improved Detection System for Whole Column Detection in Capillary Isoelectric Focusing

Abstract

An improved whole capillary scanning device was constructed which permitted die scanning of capillaries. This device allowed examination of electropherograms during the course of development, as well as eliminated the need to handle the capillaries after separation. The scanning device consisted of a detector constructed around the capillary, which was laterally driven by a DC motor via a rubber belt along polished steel rail. This design produced noise levels better than die earlier prototype on a separation of test proteins. The S/N ratio is close to that of static detection, provided a bare fused silica capillars is scanned. UV transparent capillaries still produced unacceptable noise levels, probably due to optical inhomogeneities in the outer coating which exaggerated minor positional changes due to vibration.