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Abstract
Reverse-phase HPLC methodology was developed for resolution and quantitative analysis of dicamba (3,6-dichloro-2-methoxybenzoic acid) with a C-18 column and acetonitrile-phosphate buffer as the mobile phase under isocratic conditions. This solvent system was also suitable for mixtures containing dicamba, 2,4-dichlorophenoxyacetic acid (2,4-D), and 2-(2-methyl-4-chlorophenoxy)propionic acid (MCPP) and allowed the resolution of these herbicides with the respective retention times of 4.5, 7.1, and 9.5 min. UV-spectroscopy was unsuitable for the resolution of mixtures of dicamba, 2,4-D, and MCPP due to overlapping absorption spectra.