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Abstract
Biogenic amines in Nematoda have previously been found by means of high-performance liquid chromatography (HPLC) equipped with various detectors. The methods of sample preparation have differed widely. In this study, we tested the influence of experimental conditions (temperature and duration of exposure to light, centrifugation or individual recovery by pipetting each worm, and saccharose gradient versus physiological saline) on catecholamines and indolamines content in Nippostrongylus brasiliensis at various developmental stages (larvae and adults). Amines were determined by liquid chromatography with electrochemical detection.
To the best of our knowledge, octopamine, tyramin, N-acetylserotonin and N-acetyldopamine were found and determined for the first time in this nematode.
All experimental conditions listed above modify the endogenous amine levels in larval and adult Nippostrongylus brasiliensis. This should be taken into account in the design of future research protocols.