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Abstract
A high-performance liquid chromatography method for the analysis of propylthiouracil in plasma was validated. Methylthiouracil was used as the internal standard. A one step extraction procedure and an isocratic HPLC method with UV detection were used. No plasma components were found to interfere in the assay. Linear calibration (r2 > 0.99) curves using water and plasma as matrices in the range of 0.05 to 15 μg.mL−1 were obtained. Good recoveries for propylthiouracil (> 85%) and methylthiouracil (> 65%) were seen both in water and in plasma. The coefficient of variation for repeatability was < 7%, for reproducibility < 8% and for accuracy <6%. The limit of detection was 2 ng.mL−1 in water and 5 ng.mL−1 in plasma. A poor stability of propylthiouracil was observed even at −20°C and it is recommended to perform the analyze shortly after sampling.