Abstract
A fully automated high-performance liquid chromatography method with fluorimetric detection is described for the determination of CGP 48 933 in human plasma. Liquid-solid extraction was performed automatically on C8 reversed phase column using the Gilson ASPEC system. The on-line chromatography was performed on a ODS Hypersil C18 5 μm column. The mobile phase, acetonitrile- pH 2.8 phosphate buffer (50:50, v/v) was used at a flow rate of 1.3 ml/min. The fluorimetric excitation and emission wavelengths were set at 265 and 378 nm, respectively. The limit of quantitation of CGP 48 933 was 11.5 nmol per litre of plasma.