Abstract
The interaction of propranolol with pure human α1-acid glycoprotein as well as that of bilirubin with complete human serum was studied using high-performance liquid chromatography. Hummel and Dreyer (equilibrium) size exclusion as well as (nonequilibrium) zonal elution chromatographic methods were applied. The binding interaction data were evaluated according to three different approaches introduced by Scatchard, Bjerrum, and by Tobler and Engel.
Procedure schedules designed for the study and evaluation of the reversible (equilibrium) macrobiomolecule-ligand interaction are discussed and critically commented.