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Abstract
The Resolvosil BSA-7, Chiracel OD, and Chiralpak AD columns were examined to determine whether one might be suitable for the separation of enatiomers of the ligand hexamethyl-propyleneamine oxime (HM-PAO) and/or its technetium-99m complex. Using hexane/IPA as eluent, partial separation (Rs = 0.96) of the d- and l-ligand enantiomers was achieved on the OD column, but as the meso-form of the ligand was eluted midway between the d- and l-enantiomers. There was excellent separation of the technetium-99m complexes of d- and 1-HM-PAO on the OD column, and partial separation (Rs = 0.90) of the meso- and d-complexes. Only partial resolution of the Tc-99m complexes of d,l- and meso-diastereoisomers was achieved on the AD column, but this column provided baseline resolution of all three stereoisomeric forms of the ligand. Thus, the OD column could be used for HPLC analysis of Tc-99m complexes of HM-PAO stereoisomers, while the AD column is suitable for analysis of the ligand stereoisomers.