High Performance Liquid Chromatographic Separation of Nonamidated and Glycine- and Taurine-Amidated Bile Acid 3-Glucosides

Abstract

The high-performance liquid chromatographic separation of the 3-glucosides of nonamidated lithocholic, chenodeoxycholic, ursodeoxycholic, deoxycholic and cholic acids, and their double conjugate forms with glycine and taurine has been carried out on a C₁₈ reversed-phase column. Satisfactory separation not only of each of the three groups of the nonamidated and amidated bile acid 3-glucosides but also of the individual compounds in the same group was attained by employing acetonitrile-0.3% potassium phosphate buffer (pH, 7.0) as mobile phase. The retention data reported here provide an insight into structural elucidation of these biologically important bile acid 3-glucosides.