Abstract
Mercury-substituted chlorophylls, i.e., (pheophytin a)mercury(ll) and (pheophytin b)mercury(ll), were separated by reversed-phase HPLC with a C18-bonded silica column and a mobile phase of acetone-methanol. Their spectroscopic and chromatographic characteristics strongly suggest that both (pheophytin a)mercury(ll) and (pheophytin b)mercury(ll) form 1: 1 (metal ion: chlorin ring) complexes.