Stereoselective Bioanalysis of Oxcarbazepine and the Enantiomers of Its Metabolites by High-Performance Liquid Chromatcgraphy

Abstract

The simultaneous liquid chromatographic determination of oxcarbazepine and the enantiomers of its metabolites 10,11-dihydro-10-hydroxycarbamazepine and trans-10,11-dihydroxycarbamazepine in spiked human plasma is described. The compounds are subjected to solid phase extraction before chromatography. The separation of the analytes is achieved using chiralcel OD column coupled on line with chiralcel ODH column and a mobile phase consisting of n-hexane-ethanol (70/30, v/v). The compound were detected by ultraviolet absorbance at 220 nm. The limit of quantification for each compound was 5 ng/ml.