Separation of the Two Major Anthocyanins from Champagne Vintage By-Products by Gradient Elution Centrifugal Partition Chromatography

J. H. Renault Laboratoire de Pharmacognosie URA CNRS 492 Faculté de Pharmacie 51 rue Cognac-Jay, 51096, Reims, Cedex, France

P. Thepenier Laboratoire de Pharmacognosie URA CNRS 492 Faculté de Pharmacie 51 rue Cognac-Jay, 51096, Reims, Cedex, France

M. Zeches-Hanrot Laboratoire de Pharmacognosie URA CNRS 492 Faculté de Pharmacie 51 rue Cognac-Jay, 51096, Reims, Cedex, France

A. P. Foucault Laboratoire de Bioorganique et Biotechnologies ERS CNRS 71, ENSCP 11 rue P&M Curie, 75231, Paris, Cedex, 05, France

Abstract

Centrifugal Partition Chromatography (CPC) was applied, for the first time, to the preparative scale separation of anthocyanins. The two major anthocyunins from Champagne vintage by-products, malvidin 3-glucoside and peonidin 3-glucoside, were isolated in one step, using gradient elution with the TFA-acidified ternary solvent system ethyl acetate / 1-butanol / water.

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Separation of the Two Major Anthocyanins from Champagne Vintage By-Products by Gradient Elution Centrifugal Partition Chromatography

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Separation of the Two Major Anthocyanins from Champagne Vintage By-Products by Gradient Elution Centrifugal Partition Chromatography

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