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Abstract
Fractionation of various leukemic cells in aqueous two-phase systems, composed of poly(ethylene glycol) and dextran, was performed in a high speed counter-current chromatography (HSCCC) device with eccentric parallel coil orientation. Cells from different lineages or different culture conditions showed different elution patterns. Due to the instability of the stationary phase and the tendency of cells to concentrate at the phase interfaces, special modifications of HSCCC operation were developed to give satisfactory fractionation, these include optimal timing for sample injection, application of downward concentration gradient of mobile phase fluid and proper rotational speed.