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Abstract
The present paper describes a modified high-performance liquid chromatographic (HPLC) method for the determination of thiamphenicol (THA) residues in spiked milk at levels as low as 30 ppb. Milk spiked with THA was extracted with ethyl acetate. Following addition of hexane, the extract was cleaned up with a silica cartridge. Analysis was performed on a reversed-phase C18, 5 μm, column using water-methanol (70:30) as mobile phase. Recovery was found to range from 68.0 to 90.0 % and precision data suggested that relative standard deviation ranged from 7 to 9.4 %.