Three-Dimensional Electropherogram for the Separation of Oligodeoxy-Nucleotides and DNA Restriction Fragments Using Capillary Gel Electrophoresis with a Photodiode Array Detector

Abstract

A photodiode array detection system was applied to capillary gel electrophoresis and gave reliable spectra of single-stranded oligodeoxynucleotides and double-stranded DNA restriction fragments even in the presence of high UV background caused by polyacrylamide within the capillary. Photodiode array detection enabled accurate characterization of separated DNA fragments and was a powerful tool for the identification of oligodeoxynuelcotides.