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Abstract
A reversed-phase HPLC methods in combination with Diode Array Detection is described for separation of polysaccharides in acid hydrolysates from many different agar-type polysaccharides. Temperature is optimized at 30°C. The detection limit is about 5 pmol. Sugars show a linear response in a wide concentration range. This technique is able to resolve the different oligomers according to the respective degree of polymerization (DP) in a very rapid, easy, reliable and reproducible way.