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Abstract
Anion exchange liquid chromatographic methods are described for the determination of N-phosphonomethyl-glycine (PMG), which is known also as glyphosate, its monomethylester (PMG-Met), N,N-bis(phosphonomethyl)-glycine (bis-PMG), N-methyl, N-phosphonomethyl-glycine (PMG-NMet) and glycine (Gly). Five different anion exchange columns were tested for the separation of the above mentioned compounds. Effects of the eluent pH, ionic strength and organic solvent composition were studied. It was found that only some of the tested columns were applicable for the separation of the PMG and PMG-NMet critical pair, which may be due to the different structure, the ratio of the hydrophobic and hydrophilic surface area of the ion exchanger. Analytical performance of the methods was investigated. Some examples are also shown for the application of these described methods.