![Sample our Physical Sciences journals, sign in here to start your FREE access for 14 days]({"type":"image" , "src" : "/sda/110819/TOC-Subject-Sample-2021-Physical-Sciences.jpg"})
Abstract
A simple, sensitive, and reproducible HPLC method has been developed for the determination of theophylline and zidovudine (AZT) employing reverse phase high performance liquid chromatography with UV detection at 270 nm. The separation was performed on a Novapak C18, 5 μm (3.9 × 150 mm) column. Acetonitrile (7.5%) in 0.2% acetic acid was used as the mobile phase and the run time was 8 min. Each drug was used as an internal standard for the other. The mean retention times of theophylline and zidovudine were 3.4 and 6.0 min, respectively. Linear response (r > 0.998) was observed over the range of 0.02–10 μg/ml for both drugs. There was no significant difference (p < 0.05) between inter- and intra-day studies for theophylline and zidovudine. The mean relative standard deviations (RSD%) of the results of within-day precision and accuracy of the two drugs were < 7%. The applicability of the assay was demonstrated in determining each drug concentrations in different groups of rats.