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Abstract
The determination of free amino acids in some human body fluids using reversed phase high performance liquid chromatography and pre-column derivatization with ortho-phthaldialde-hyde/mercaptoethanol is a highly sensitive and reproducible method. However not all amino acids can be determined using this easily automatizable method. Several special amino acids, as for example cystin which contains sulphur, can be determined using a separation procedure of ion-exchange and/or ion-pair chromatography. These separation procedures are described here.