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Abstract
2,6-Dideoxysugars, biosynthetically derived from glucose through sugar nucleotide intermediates, are important structural components of antibiotics. A HPLC method was developed for the detection of ADPG, UDPG, CDPG, GDPG and/or dTDPG in cell free extracts of Streptomyces griseus. The resolution of these sugar nucleotides and fourteen additional related mono-, di- and triphosphoribo-nucleotides was achieved by gradient elution, ion-pair reversed-phase chromatography (RP-IPC) over an ODS-C18 column. Cell free extracts contained UDPG, GDPG and UDP, thus implicating the two sugar nucleotides in chromomycin-A3 biosynthesis.