Determination of Dextromethorphan Hydrobromide in Bulk Form and Dosage Formulations by High Performance Liquid Chromatography

Abstract

A simple LC-procedure for quantification of dextromethorphan in pure forms and pharmaceutical preparations (tablets, syrups, and drops) is described. The HPLC-separation of the active ingredient from the complex matrices of the dosage formulations is undertaken by dilution or extraction in the mobile phase [acetonitrile/acetate buffer (40 mM, pH 4.3) = 75:25, v/v] and elution on a reverse-phase μ-Bondapak™ C₁₈ column (30 cm × 3.9 mm φ, 10 μm) isocratically (1.5 ml. min⁻¹) with UV-detection at 278 nm at ambient temperature. Good recovery testing of drug masses added to the dosage forms was obtained.