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Abstract
A rapid, specific and reliable high performance liquid chromatographic assay of Nalidixic acid in tablets has been developed. Reversed-Phase chromatography was conducted using a mobile phase of 0.05 M Ammonium acetate Methanol and acetonitrile, (65, 5, 30% v/v) pH 5 and detection at λ 254 nm. The recovery and coefficient of variation from six placebo tablets containing 100 mg of Nalidixic acid were 100.2% and 0.56 respectively. Replicate regression analyses of three standard plots in the concentration range 1–20 mcg/ml obtained on three different days gave a conelatioil coefficient (0.99996) and the coefficient of variation of the slopes 0.089%. The assay was precise within day and between days as indicated by ANOVA test. It is suggested that the proposed HPLC method should be used for routine quality control and dosage fonn assay of Nalidixic acid.