HPLC Determination of Propofol-Thiopental Sodium and Propofol-Ondansetron Mixtures

Abstract

High performance liquid chromatography procedures have been developed for the assay of propofol-thiopental sodium and propofol-ondansetron mixtures. The separation and quantitation of propofol-thiopental sodium were performed on a stable bond phenyl column at ambient temperature using a mobile phase of 55:45 v/v aqueous 0.01 M monobasic postassium phosphate pH 4 - acetonitrile at a flow rate of 1 mL/min, with detection set at 235 nm. The separation was achieved within 20 min. Propofol and thiopental sodium were linear in the 12.7 - 38 and 31.4 - 94 μ/mL ranges, respectively. Accuracy and precision were in the range 0.2 - 2.6 and 0.2 - 3.2%, respectively, for the two analytes and the limits of detection for propofol and thiopental sodium were 1210 and 317 ng/mL, respectively, based on a signal to noise ratio of 2 and a 20 μL injection. The separation and quantitation of the propofol-ondansetron mixture was achieved on a 10 μm particle size phenyl column., using a mobile phase of 50:50 v/v aqueous 0.01 M monobasic potassium phosphate pH 4 - acetonitrile at a flow rate of 1 mL/min, with detection set at 268 nm. The separation was achieved within 15 min. Propofol and ondansetron were linear in the 2.5 - 37.5 and 0.48 - 7.2 μg/mL ranges, respectively. Accuracy and precision were in the range 0.4 - 2,4 and 0.2 - 0.6%, respectively, for the two analytes and the limits of detection for propofol and ondansetron were 117 and 61 ng/mL, respectively, based on a signal to noise ratio of 2 and a 20 μL injection.