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Abstract
Tetrahydrobiopterin and seven of its analogues (neopterin, xanthopterin, biopterin, pterin, tetrahydropterin, 6-methyltetrahydropterin, and 6-methylpterin) were separated on a 1 × 150 mm C18 microbore column. These analytes were detected by dual-electrode amperometry and UV absorption. Both a conventional glassy carbon electrode and an interdigitated array microelectrode were used. Low fmol amounts of xanthopterin, tetrahydropterin, tetrahydrobiopterin and 6-methyltetrahydropterin could be determined by electrochemical detection in the oxidative mode, but pmol amounts of the other analogues were determined by electrochemical detection in the reductive mode and with UV detection.
Catecholamines and their metabolites do not interfere with the determination of tetrahydrobiopterin and its analogues in biological samples. The developed method was explored for the determination of tetrahydrobiopterin in samples of human urine and rat tissue (brain, liver, and kidney).