Capillary Electrophoretic Enantiomeric Separations of Nonsteroidal Anti-inflammatory Compounds Using the Macrocyclic Antibiotic Actaplanin A and 2-Methoxyethanol

Abstract

Actaplanin A, a macrocyclic antibiotic, was examined as a chiral selector in capillary electrophoresis (CE) for the enantioseparation of several racemic nonsteroidal antiinflammatory compounds. The chiral selectivity of this macrocyclic antibiotic was evaluated as a function of the run buffer pH, chiral selector concentration, and organic modifier composition. Optimized conditions using 15-30% of 2-methoxyethanol and 0.5 mM actaplanin A in 40 mM phosphate buffer (pH 6) were successful in separating all of the enantiomers from the racemic compounds tested in this study.