Determination of 1-Octanol-Water Partition Coefficients for a Group of 1,4-Dihydropyridines by Reversed-Phase High Performance Liquid Chromatography: Study of Micellar Liquid Chromatography for Hydrophobicity Estimation

Abstract

1-Octanol-water partition coefficients (P_ow) were determined for a group of 1,4-dihydropyridines by the classical extraction method called “shake-flask” method. Reversed-Phase High Performance Liquid Chromatography (RP-HPLC) was employed to determine the concentration of each solute in the aqueous phase after equilibrium. Micellar Liquid Chromatography (MLC) was used to obtain chromatographic parameters (k′, logk′, k′_m, logk′_m, P_mw, and logP_mw) which were tested as hydrophobicity descriptors for the dihydropyridines studied. The correlation of these parameters with logP_ow was studied. Sodium Dodecyl Sulphate and Hexadecyltrimethylammonium Bromide modified by n-propanol or n-butanol were used as micellar systems in MLC.