Abstract
High performance liquid chromatographic methods have been developed for the assays of mixtures containing doxorubicin-morphine, doxorubicin-granisetron, and doxorubicin-cyclophosphamide in 0.9% sodium chloride injection USP. The separation and quantitation of each mixture were achieved on octylsilane columns at ambient temperature using a mobile phase of aqueous sodium dihydrogen phosphate - acetonitrile at a flow rate of 1.0 mL/min with UV detection. The separations were achieved within 13 min. The methods showed linearity for the analytes in their respective calibration ranges. Accuracy and precision were in the 0.1–3.3% and 0.2–3.1% ranges, respectively, for the various analytes.