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Abstract
A new kind of centrifugal countercurrent partition chromatographic column has been developed. The shape of the column looks like the chain of a bicycle. Using the column, retention ratio of the stationary phase was measured with three kinds of solvent systems under various experimental conditions. The chloroform solvent systems showed retention from 52 to 66% when the upper phase was used as the stationary phase. The performance of the column has been successfully demonstrated in the separation of dinitrophenyl (DNP) amino acids, Weimeisu and an extract of branch-leaves of Taxus cuspidate.