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Abstract
A novel reversed phase chromatography packing to be used for analytical protein separations is described. A macroporous silica support was bonded with diethoxymethylvinyl silane, and then copolymerized with hydroxyethyl methylacrylate and divinylbenzene to produce tailored stationary phase. This packing has high resolution and stability in extent pH range. It was characterized through the separation of two model protein mixtures, the first one containing lysozyme, cytochrome C, β-lactoglobulin, chick egg albumin, ribonuclease and pepsin (porcine mucus), the second mixture containing insulin (two kinds), ribonuclease, lysozyme, serum bovine albumin. Such manipulations of proteins' elution profile are achievable at run times of less than 15 minutes. It is suggested that the macroporous silica-based polymer-bonded packing is a better alternative for the biopolymer separation.