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Abstract
A simple high performance liquid chromatographic method for the determination of oxolinic acid, a chemotherapeutic agent, in turbot serum has been developed. Nalidixic acid was used as an internal standard. The drugs were separated by using a 5 μm Supelcosil ABZ+Plus® reversed phase cartridge, a mobile phase of acetonitrile, tetrahydrofuran and 0.001 M orthophosphoric acid solution (22:10:68 v/v), pH 3.4 with a fluorescence detection. The sample clean-up was reduced to the use of precipitating agents and centrifugations because the solid-phase extraction procedure was not suited to the analysis of oxolinic acid in turbot serum.
Linearity and precision were checked over the concentration range 0.020–2.500 μg/mL. Limits of detection and determination were respectively 0.005 and 0.015 μg/mL. Recoveries of oxolinic acid from turbot serum were between 103.89 and 110.71 %.