Purification of Anti-HIV Lignans from Larrea tridentata by pH-Zone-Refining Countercurrent Chromatography

Y. Ma Laboratory of Biomedical Chemistry, National Heart, Lung, and Blood Institute, National Institutes of Health Bldg. 10, Rm 7N322 10 Center Drive MSC 1676, Bethesda, MD, 20892-1676, USA

L. Qi Laboratory of Biomedical Chemistry, National Heart, Lung, and Blood Institute, National Institutes of Health Bldg. 10, Rm 7N322 10 Center Drive MSC 1676, Bethesda, MD, 20892-1676, USA

J. N. Gnabre Department of Biology, The Johns Hopkins University, 114 Mudd Hall 3400 N. Charles Street, Baltimore, MD, 21218-2685, USA

R. C. C. Huang Department of Biology, The Johns Hopkins University, 114 Mudd Hall 3400 N. Charles Street, Baltimore, MD, 21218-2685, USA

F. E. Chou Pharma-Tech Research Corporation, 6807 York Avenue, Baltimore, MD, 21212, USA

Y. Ito Laboratory of Biomedical Chemistry, National Heart, Lung, and Blood Institute, National Institutes of Health Bldg. 10, Rm 7N322 10 Center Drive MSC 1676, Bethesda, MD, 20892-1676, USA

Abstract

Anti-HIV lignans were purified from extract of Larrea tridentata by high-speed countercurrent chromatography (CCC) using pH-zone-refining CCC. When a column filled with methyl t-butyl ether, containing trifluoroacetic acid at 25 mM, was eluted with aqueous NaOH, 10 to 20 g of the crude extract was separated into NDGA (nordihydroguaiaretic acid) and its monomethyl esters rectangular peaks associated with their specific pH (pH zones). The method was also successfidly applied to synthetic lignans, resulting in resolution of NDGA and its mono and dimethyl esters.

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Purification of Anti-HIV Lignans from Larrea tridentata by pH-Zone-Refining Countercurrent Chromatography

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Purification of Anti-HIV Lignans from Larrea tridentata by pH-Zone-Refining Countercurrent Chromatography

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