High-Performance Liquid Chromatographic Determination of Mn(II), Co(II), Zn(II), Ni(II), Cu(II) As Coproporphyrin-I Complexes

Abstract

The mobilities of 3,8,13,18-tetrametyl-21H,23H-porphine-2,7,12,17-tetrapropionic acid or coproporphyrin-I (CPI) and five metal-CPI complexes were measured by reversed-phase high performance liquid chromatography (RP-HPLC) on a octadecyl-bonded silica gel (C₁₈ silica) with developing solvent (69:31 v/v) methanol-buffer at pH 7 (buffer ionic strength 0.270 mol L⁻¹. 0.018 mol L⁻¹ boric, phosphoric and acetic acid, and 0.036 mol L⁻¹ sodium hydroxide), and 0.050 mol L⁻¹ of NaCl, with UV detection at 374 and 458 nm.