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Abstract
High-speed countercurrent chromatography (HSCCC) was applied to the separation and purification of 2,3,5,4′-tetrahydroxy stilbene-2-O-D-glucoside from dried roots (2.7g) of Polygonum multiflorum. The ethanol extract (240 mg) was first separated with the solvent system composed of ethyl acetate-ethanol-water at a volume ratio of 10:1:10 and the obtained fraction was repurified with the solvent system at the modified volume ratio of 50:1:50. The method produced stilbene glucoside (30mg) at a high purity of 96%. The chemical structure was identified by FAB-MS and 1HNMR.