Compositional Analysis of the Major Capsular Polysaccharides of Cryptococcus Neoformans by Highperformance Anion-Exchange Chromatography with Pulsed Amperometric Detection (HPAEC-PAD)

C. Corradini Istituto di Cromatogrdia C.N.R. Area della Ricerca di Roma, P.O. Box 10, Monterotondo Stazione, 1-00016, Italy

G. Canali Istituto di Cromatogrdia C.N.R. Area della Ricerca di Roma, P.O. Box 10, Monterotondo Stazione, 1-00016, Italy

A. Cavazza Istituto di Cromatogrdia C.N.R. Area della Ricerca di Roma, P.O. Box 10, Monterotondo Stazione, 1-00016, Italy

D. Delfino Universita degli Studi di Messina, Istituto di Microbiologia FacoltA di Medicina e Chirurgia, Messina, 1-98122, Italy

G. Teti Universita degli Studi di Messina, Istituto di Microbiologia FacoltA di Medicina e Chirurgia, Messina, 1-98122, Italy

Abstract

Cryptococcus neoformans is a predominantly saprophytic yeast that can cause serious infections, mostly in individuals with acquired immunodeficiency syndrome (AIDS).

A prominent virulence factor of Cryptococcus neoformans is its capsule. This is constituted mainly of glucans; the capsular polysaccharide, glucuronoxylomannan (GXM) (composed of mannose, xylose, glucuronic acid), and at least two minor carbohydrate antigens, galactoxylomannan (GalXM) and mannoproteins (MP).

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Compositional Analysis of the Major Capsular Polysaccharides of Cryptococcus Neoformans by Highperformance Anion-Exchange Chromatography with Pulsed Amperometric Detection (HPAEC-PAD)

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Compositional Analysis of the Major Capsular Polysaccharides of Cryptococcus Neoformans by Highperformance Anion-Exchange Chromatography with Pulsed Amperometric Detection (HPAEC-PAD)

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