Determination of Blood Glucose by Quantitative TLC on Preadsorbent Silica Gel Plates

Abstract

A thin layer chromatography method was developed for quantification of glucose in human blood serum using preadsorbent silica gel plates, acetonitrile-water (85:15) mobile phase, methanolic sulfuric acid detection reagent, and scanning densitometry. Results are reported for the measurement of glucose by the TLC method before and after consumption of a sucrose solution. The method was validated by determining the precision for replicate analyses and accuracy by analyzing a spiked sample and also comparing results with those given by a commercial kit intended for use by diabetics for home monitoring of glucose levels. The TLC method is simple to perform and can serve for the analysis of other sample types and as an interesting experiment illustrating quantitative TLC in college analytical chemistry or biochemistry laboratory courses.