Removal of 1,3-Dichloro2-Propanol and 3-Chloro1,2-Propanediol by the Whole Cell System of Pseudomonas putida DSM 437

The removal of 1,3-dichloro-2-propanol (1,3-DCP), 3-chloro-1,2-propanediol (3-CPD) and their mixtures at concentrations up to 1000 mg · L⁻¹ by the whole cell system of Pseudomonas putida DSM 437 was investigated. The 1,3-DCP removal rates ranged from 2.36 to 10.55 mg · L⁻¹ · h⁻¹; 3-CPD exhibited approximately two times higher removal rates compared to 1,3-DCP for all concentrations tested. Removal of 1,3-DCP and 3-CPD followed first-order kinetics with rate constants of 0.0109 h⁻¹ and 0.0206 h⁻¹, respectively. When the whole cell system of P. putida DSM 437 was applied to mixtures of the two halohdrins, complete removal of 1,3-DCP was achieved at 144 h while removal of 3-CPD was completed at times ranging from 72 to 144 h. Time to achieve 50% removal of both halohydrins depends on the initial concentration of each in the mixture. For 1,3-DCP, it ranged from 40.55 h at 200 mg · L⁻¹ to 53.28 h at 500 mg · L⁻¹ while the respected values for 3-CPD were 33.39 and 68.91 h.

Key Words:

• Pseudomonas putida
• Whole cell
• Removal
• 1,3-Dichloro-2-propanol
• 3-Chloro-1,2-propanediol.

Notes

^a R ² = 0.99, Standard Error of Estimate = 3.6, F = 2916.1, P < 0.0001.

^b R ² = 0.99, Standard Error of Estimate = 9.1, F = 903.8, P < 0.0001.

^c R ² = 0.99, Standard Error of Estimate = 3.1, F = 18554.7, P < 0.0001.

^d R ² = 0.99, Standard Error of Estimate = 3.8, F = 17759.4, P < 0.0001.

^a R ² = 0.99, Standard Error of Estimate = 4.8, F = 1623.9, P < 0.0001.

^b R ² = 0.99, Standard Error of Estimate = 10.6, F = 769.1, P < 0.0001.

^c R ² = 0.99, Standard Error of Estimate = 3.3, F = 17595.3, P < 0.0001.

^d R ² = 0.99, Standard Error of Estimate = 20.7, F = 520.6, P < 0.0001.