Antioxidant status and selected biochemical parameters of porcine ovarian granulosa cells exposed to lead in vitro

Abstract

The objective of this study was to determine the activity of superoxide dismutase (SOD), total antioxidant status (TAS) and release of calcium, phosphorus, magnesium, sodium, potassium, total lipids, totals proteins, glucose, cholesterol and triglycerides by porcine ovarian granulosa cells cultured in vitro after lead acetate administration. The parameters were analyzed using semi–automated clinical chemistry analyzer Microlab 300, microprocessor-controlled analyzer EasyLite and spectrophotometer Genesys 10. Cells were cultured with lead acetate trihydrate [Pb(CH₃COO)₂.3H₂O] as follows: group Max (5 mg Pb(CH₃COO)₂.3H₂O/10 mL), group A (2.5 mg/10 mL), group B (0.83 mg/10 mL), group C (0.625 mg/10 mL), group D (0.455 mg/10 mL) and the control group without lead exposure for 18 hrs. The highest TAS was estimated in the control group without lead treatment in comparison with other groups (MAX, A, B, C, D). Statistical analyses showed significantly lower value (P < 0.05) in group B. The activity of SOD was the lowest in the control group in comparison to those exposed to in vitro lead culture. A significant decrease (P < 0.05) of calcium content in group MAX in comparison with control group was determined. Release of phosphorus by ovarian granulosa cells was significantly lower (P < 0.05; 0.01; 0.001) in all the treated groups in comparison with control group. Lead was found to stimulate the release of magnesium and potassium by granulosa cells, but the increase remained statistically insignificant. The highest concentration of glucose was noted in control group, but the differences were not significant either. No significant differences (P > 0.05) were detected in concentration of other studied parameters among observed groups, too.

Keywords:

• porcine ovarian granulosa cells
• lead
• antioxidant
• biochemistry

Acknowledgments

The authors are thankful to Ing. Peter Cupka, Ing. Monika Schneidgenova and Viera Bosel for skillful technical assistance. This work was financially supported by VEGA scientific grant 1/0696/08.

Notes

*Denotes significant differences from control (P < 0.05;

** P < 0.01;

*** P < 0.001), other differences were not significant (P > 0.05).