Comparisons of Vibrio fischeri, Photobacterium phosphoreum, and recombinant luminescent using Escherichia coli as BOD measurement

Abstract

To shorten the time needed to measure biochemical oxygen demand (BOD) in water samples and to provide a rapid feedback of the real condition of water quality, we tested and evaluated the validity and reliability of luminescent bacteria Vibrio fischeri, Photobacterium phosphoreum, and recombinant Escherichia coli as potential indicators of BOD in the domestic wastewaters. The results indicate that the luminescence intensities of these strains are dependent on temperature, pH, and BOD concentration. In comparison to the standard BOD₅ method, the time needed for BOD measurement can be shortened by 90, 120, and 150 min when V. fischeri, P. phosphoreum, and recombinant E. coli, respectively, are used. Recombinant E. coli can be adapted to measure BOD in domestic wastewater containing a wide range of BOD concentrations, V. fischeri is not suitable for measuring diluted wastewater, and P. phosphoreum has only a limited application in measuring concentrated wastewater. To the best of our knowledge, this is the first report in which V. fischeri, P. phosphoreum, and recombinant luminescent E. coli are compared in terms of their potential in BOD measurement systems.

Keywords:

• Biochemical oxygen demand
• biosensor
• Vibrio fischeri
• Photobacterium phosphoreum
• Escherichia coli

Acknowledgments

The work was supported by Grant NSC 97-2815-C-157-002-E from the National Science Council.

Notes

∗Data were compared with data measured by the standard BOD₅ method.